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E harvests for real-time PCR analysis of Egr2. All real-time PCR results are from two separate experiments performed in triplicate and results were normalized to amplification of ACTB mRNA. Bars represent mean ?SEM and are expressed as fold change with respect DMSO treated cells. *p
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On cell number) was used for cytospin preparation. Slides were air-dried for 30 min, fixed O/N in 10 formalin, then stored in 70 ethanol at 4 , until IHC staining. IHC staining was performed using Ventana Benchmark ULTRA automated slide stainers, and antibodies against pan keratin (Neomarkers, Fremont,CA, USA, MS-343-P), EpCAM (DAKO, Heverlee, Belgium, M0804) and p53 (DAKO, M7001).Pathway ana
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On cell number) was used for cytospin preparation. Slides were air-dried for 30 min, fixed O/N in 10 formalin, then stored in 70 ethanol at 4 , until IHC staining. IHC staining was performed using Ventana Benchmark ULTRA automated slide stainers, and antibodies against pan keratin (Neomarkers, Fremont,CA, USA, MS-343-P), EpCAM (DAKO, Heverlee, Belgium, M0804) and p53 (DAKO, M7001).Pathway ana